Mother of Vinegar: Healthy and Probiotic? A Difficult Question Explored

Reginald SmithAll About Vinegar, Health & Wellness, Mother of Vinegar12 Comments

White wine mother

As a vinegar manufacturer, it is my job to be ‘hip’ on the trends and demand in the market for vinegar, not just in the USA but globally. One of the biggest trends to shake up the vinegar industry in the last 10-15 years is the popularity of raw and unfiltered vinegar, usually with copious amounts of mother of vinegar included in the bottle.

This reverses almost 100 years of practice starting in the late 19th century when pasteurization and filtering were the signs of the modernity and quality of a vinegar establishment. Vinegar is unique though. Its strong antimicrobial properties make pasteurization unnecessary to preserve freshness. Vinegar does not spoil in the traditional sense. Pasteurization is primarily to eliminate the acetic acid bacteria, which make vinegar, and prevent the mother from forming which past consumers found aesthetically unpleasant.

Filtration and pasteurization do also adjust the flavor profile so there is a trade off. Much the excitement about raw and unfiltered vinegar is the supposed additional health benefits. As a vinegar maker I am definitely happy to have any selling point possible and many customers contact me asking if a vinegar is “raw”. However, I also want to understand if there really are any benefits and if so, what they are.

Overall, there are indeed many health benefits to vinegar. This is not a New Age or food snob rant. It is backed up by scientific studies going back 30 years and recently wonderfully organized and catalogued in a new peer-reviewed journal article by Chen et. al. (see bottom of article). There are many benefits including antioxidant properties, blood glucose reduction and the moderation of lipds (cholesterol). Many of these benefits are provided by the actions of acetic acid and other organic acids in vinegar. Others, like antioxidant properties, are provided by compounds called polyphenols which vary depending on the type of vinegar and whether it is aged in wood barrels. One of the co-authors of the review paper is Paolo Giudici, arguably one of the top researchers on vinegar in the world and editor of the magnum opus of vinegar scientific and industrial knowledge, Vinegars of the World.

The mother of vinegar itself has been much less investigated. In fact there is only a sole paper focusing on its health properties and benefits which will be discussed later.

As stated in an earlier post, the mother of vinegar is what is technically called a biofilm. It is primarily made of cellulose, the same thing as grass and most plant structural material. For humans, cellulose is undigestible, though it is a great source of fiber for bowel health. The mother of vinegar cellulose has a different structure that gives it its gooey appearance. It is still cellulose though and may one day provide us with an alternate source for paper via transgenic acetic acid bacteria. The mother exchanges oxygen and water with the atmosphere and provides a nice environment for acetic acid bacteria to thrive. Though bacteria are the makers of vinegar, the genuses of acetic acid bacteria are all non-pathogenic*.

There are a few main ways the mother might be healthy. One, the increased concentration of bacteria may have a probiotic effect in the body. Two, the cellulose in the mother, though indigestible, may provide the same benefits as fiber intake. Three, there could be special compounds in the mother that have known health effects.

Probiotic Vinegar Bacteria?

First, let’s look at probiotics. Many of the popular probiotic products are aimed at helping to replenish helpful gut bacteria that live in symbiosis with us and provide health benefits such as aiding in digestion and pushing out potentially harmful bacteria. The probiotic yogurts, kefir, etc. are legion. Do acetic acid bacteria play this role though?

Acetic acid bacteria need oxygen to survive. They use it to metabolize alcohol to acetic acid to make vinegar and die without it. They are thus obligate aerobes: they need oxygen to survive. Obviously in our guts, oxygen is in short supply. Other bacteria that are probiotic like lactic acid bacteria are anaerobes and survive in the absence of oxygen so live fine in our gut. Indeed, it seems acetic acid bacteria are not probiotic–at least as far as the human gut is concerned.

Two studies on the communities of bacteria in the human gut (see the references of Gill and Qin at the end of the article) used metagenomic analysis, a method to analyze the organisms in a sample by DNA. Of the dozens of bacteria genuses they found, the acetic acid bacteria ones (Acetobacter, Gluconoacetobacter, Gluconobacter, and Komagateibacter) where absent in both studies. So this lack of evidence and the requirement for oxygen throw a bit of shade on the probiotic benefits of live vinegar bacteria for our intestinal tract.

The gut is not the only microbiome however in our bodies. Another hugely important one is the oral microbiome in our mouths. Here there is a huge database of bacteria known to exist at the Human Oral Microbiome Database (HOMD). Here again, none of the acetic acid bacteria genuses are present. Same with the skin microbiome (Grice et. al. below). In the oral cavity and skin, oxygen are not issues so if acetic acid bacteria are not present, perhaps they cannot survive there for long periods. Granted more research needs to be done and they can still pop up; many flying insects have been found to use acetic acid bacteria extensively in their microbiomes.

So until we no more, the probiotic effects of raw vinegar may be limited or nonexistent.

The Fiber Angle

I honestly won’t write much about this since I am not fully familiar with the topic. However, given it is pure cellulose, the mother of vinegar should provide the same health benefits as, for example, celery sticks. The amount that you would need to ingest, however, is likely liters of vinegar to get the same effect making raw vinegar likely not the best way to stay regular

Other Compounds in the Mother

The only scientific study thus far done on the vinegar mother by itself is one by Aykin et. al. below. In it they measure and compare the presence of many types of minerals, phenolics, and antixoidants in samples of liquid vinegar versus samples of the mother. Their results are interesting.

First, it does not seem the mother possesses any compound the vinegar itself doesn’t already have. In fact for minerals, the mother has a lower concentration of the same minerals. However for phenolic compounds like gallic acid, the concentration in the mother was often higher than the vinegar. For gallic acid, the concentration was almost an order of magnitude (10x) in the mother than the surrounding vinegar. Given the potent antioxidant properties of gallic acid and other phenols, this is a huge plus. Iron (Fe) was also found in a higher concentration in mothers as well.



The health properties of vinegar are real and increasingly validated through scientific studies. The additional benefits of the mother in raw vinegar, over and above the vinegar itself, are still under evaluation and discovery. However, some of the most widely discussed benefits, such as a gut probiotic, seem unlikely and unsupported by the evidence. It is a source of fiber, but a poor source relative to its concentration. The biggest benefit seems to be that the mother of vinegar concentrates powerful antioxidants like gallic acid in much higher concentrations than the surrounding vinegar. This seems to be the most proven benefit so far.

*There was one rare case of a pathogenic acetic acid bacteria infection though it has not seemed to be communicable and was found only in patients with compromised immune systems.


Aykın, E., Budak, N. H., & Güzel-Seydim, Z. B. (2015). Bioactive components of mother vinegar. Journal of the American College of Nutrition, 34(1), 80-89.

Chen, H., Chen, T., Giudici, P., & Chen, F. (2016). Vinegar Functions on Health: Constituents, Sources, and Formation Mechanisms. Comprehensive Reviews in Food Science and Food Safety, 15(6), 1124-1138.

Gill, S. R., Pop, M., DeBoy, R. T., Eckburg, P. B., Turnbaugh, P. J., Samuel, B. S., … & Nelson, K. E. (2006). Metagenomic analysis of the human distal gut microbiome. science, 312(5778), 1355-1359.

Grice, E. A., Kong, H. H., Conlan, S., Deming, C. B., Davis, J., Young, A. C., … & Turner, M. L. (2009). Topographical and temporal diversity of the human skin microbiome. science, 324(5931), 1190-1192.

Qin, J., Li, R., Raes, J., Arumugam, M., Burgdorf, K. S., Manichanh, C., … & Mende, D. R. (2010). A human gut microbial gene catalogue established by metagenomic sequencing. nature, 464(7285), 59-65.

12 Comments on “Mother of Vinegar: Healthy and Probiotic? A Difficult Question Explored”

  1. Hi there. I found this write up interesting since we are also experimenting on how to make a healthier vinegar from fruits.
    Do you consider mixing fruits vinegars. example:mango and grape vinegar with the coconut vinegar?
    i want to know your thought on this, please. Thank you

    1. Hi, mixing fruit vinegars could be a good way of finding a tastier vinegar and combining the health benefits of both. Much of the health benefits of vinegar come from acetic acid, which is present in all vinegars, but different vinegars do have different benefits based on their base (fruit, coconut, etc.) so combining them does leverage their benefits across types.

    1. They don’t do much harm to your gut, the vinegar is way diluted by then, but you are right that within the vinegar proper lactic acid bacteria usually do not survive.

    2. Thank you so much! Can you add anything to the value of vinegar as topical rinse for dogs skin?
      What type of harmful bacteria on dog’s skin can not survive in acetic acid? And how will results differ between raw and pasteurized vinegar?

      1. Hi I don’t have tips on pets but (by Mizkan, one of the largest manufacturers in the world) has a page on tips for pets with three links for dog tips. See here:

        Acetic acid will pretty much kill all harmful bacteria (but not necessarily viruses). Raw and pasteurized vinegar should have the same effect so get the cheaper pasteurized version.

  2. Very interesting article. Thank you. I have been looking into probiotics in Kombucha and was wondering whether the acetic bacteria in Kombucha could be classed as probiotics. Your point regarding their need for oxygen is well made. Did you ever look into compounds present in Kombucha – if so I would like to hear your thoughts on this. Thanks again for a great article.

    1. The article below describes isolates of Lactic Acid bacteria in black tea kombucha which are probiotic. As far as helpful compounds, in my opinion they would be the same compounds in black tea with the SCOBY providing the same benefits of a typical vinegar mother.

      Coton, Monika, et al. “Unraveling microbial ecology of industrial-scale Kombucha fermentations by metabarcoding and culture-based methods.” FEMS microbiology ecology 93.5 (2017).

  3. You say that your job requires you “to be ‘hip’ on the trends and demand in the market for vinegar, not just in the USA but globally.” Here is where the new trend & demand will be focused, especially in any country that allows HUGE amounts of glyphosate into our food supply. … Here is a quote from that you may find interesting: “To reduce glyphosate in your body, a study from Africa shows that acetobacter degrades glyphosate, breaking it down into nontoxic components, causing the glyphosate molecule to be gone. Acetobacter is found in Apple Cider Vinegar, sauerkraut and kombucha.” It would be well worth your time to investigate ingestion of glyphosate. Seneff, Samsel, Mercola, Bergman, & Gillam all have good info.

  4. Will the mother left on vinegar caused the vinegar to be less acidic? I have a PH meter and am finding that the PH changes ??
    Some of my vinegar makes more mother than vinegar. Why?
    I purchased device to check sugar and alcohol so I would know when to strain off my vinegar. (It doesn’t work for small amounts like gallons or less.).
    Thanks for the advice.

    1. Hello Sue. I’m certain that Reginald has a more precise methodology on the mother but I can offer you my experiences with that.

      I operated a static system for more than 30 years and studied the effects mother has on the process. Because my process was ongoing, the ratios that I fermented would produce mother at different rates. The more available sugar, the more mother production. For me, I had to develop a method that would produce “finished” vinegar before the mother production became too intense. Too much mother will hinder the oxygen supply to the Acetobacter, at the surface.

      If you have a mother that is very thick, it definitely will interfere with the oxygen take up and probably indicates, the available ethanol is used up. If this be the case, the Acetobacter is consuming the acetic acid it has already produced which means the acid value of your vinegar is decreasing. I hope I have offered some meaningful advice. I would recommend that you wait for Reginald to respond for a more precise answer to your questions.
      Roger (aka Mr. Vinegar)

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