# Temperature and Speed for Bacterial Fermentation

Reginald Smith1 Comment

Much of the food we love to ferment is fermented by bacteria. Obviously vinegar, which my company is dedicated to, is a prime example with several families of acetic acid bacteria contributing depending on the method of vinegar fermentation, acidity, and starter alcohol. For a variety of fermented foods, however, lactic acid fermentation by various lactic acid bacteria is primary. From yogurt to sourdough bread to kimchi, they are definitely one of the world’s favorite fermentation organisms.

Those who have experienced fermentation soon learn that for most types of fermentation, temperature plays a prime role in determining how fast fermentation will proceed–or if it will proceed at all. Bacteria (and yeast) are finicky and want their environment just right. Ten degrees can make the difference between an excellent and stuck fermentation so environment is crucial.

But why does temperature have the crucial role it does? Temperature can play a variety of roles in chemical kinetics, the study of the speed of chemical reactions. Usually a certain minimum temperature is needed to give molecules the “activation energy” they need to carry out a reaction. Beyond that, temperature usually speeds up the reaction the higher it gets, but there are always limits. The limits are most important in biochemical reactions which are often catalyzed by enzymes—proteins made from the DNA of the organisms that use them. At too high a temperature the reaction slows and eventually the enzyme itself becomes inactive in a process called denaturing.

When many types of yogurt are fermented, you start heating the milk to 180 F or so. This is primarily to denature certain proteins to allow them to form looser structures and assimilate well into creamy yogurt. So super high temperatures are bad for biochemical reactions. But fermentation is more than just a biochemical reaction. It is carried out by microorganisms and these organisms have their own temperature requirements. Usually their range of growth and fermentation efficiency is much narrower, and lower, than the limits of the proteins they use. The range for vinegar bacteria is best between 75 – 85 F (or 25 – 30 C). For lactic acid bacteria, it depends on the type of bacteria and fermentation. Thermophilic lactic acid bacteria for yogurt work best between 110 to 115 F. Sourdough bread lactic acid bacteria prefer the low 90s F. In almost all cases, the fermentation productivity slowly ramps up from a minimum to get to the best temperature. It then subsequently falls off a cliff if you proceed much higher than the optimum.

Fermentation productivity is related to the metabolism of the bacteria, but most importantly it is related to their reproduction rate. All things equal, faster exponential growth by cell multiplication is a bigger driver than streamlining their internal chemistry.

A typical growth curve vs. temperature, one for a common acetic acid bacteria, Acetobacter Aceti, is shown below [1].

Other bacteria like sourdough’s Lactobacillus sanfranciscensis have similar curves at different temperatures [2] based on equation from [3]

Warning: Math starts here

The growth rate on the y-axis is the growth parameter, the kind you would see in an equation for exponential growth like:

$N=N_0 e^{\alpha t}$

$N$ is the bacteria population, $N_0$ is the starting population, $t$ is time, and $\alpha$ is the growth parameter. As you see from the graph, the growth parameter is very dependent on temperature.

The rise and fall of the growth parameter with temperature is modeled in many ways but there are two that are most common. One uses an equation with two parts: a first part that governs the growth and a second that governs the mortality (death). At lower temperatures growth rules while at higher ones, mortality cuts the growth rate down sharply.

$\alpha_{max} = Ae^{\frac{-E_a}{RT}}-Be^{\frac{-E_b}{RT}}$

Yes, these are the Arrhenius equation for each part.

I won’t get too complicated right now but $A$ and $B$ are constants determined by the microorganism, $E_a$ and $E_b$ are kinetic parameters dependent on temperature for growth and death respectively. $T$ is temperature and $R$ is the gas constant, well known to those who have studied chemistry. All these have to be solved experimentally. Based on Acetobacter Aceti, the values are (from [1]) – $A=0.5 h^{-1}$, $B=8.97 x 10^7 h^{-1}$, $E_a= 417.2 J mol^{-1}$, $E_b=5974 J mol^{-1}$, $R=8.31 J K^{-1}$. Note, there seems to be a mistake in [1] such that the figure is replicated only if the temperature inserted is Celsius, not Kelvin so for the values above use Celsius for T or for Kelvin use

$\alpha_{max} = Ae^{\frac{-E_a}{R(T-273)}}-Be^{\frac{-E_b}{R(T-273)}}$

The second method from [3] was first deduced via nonlinear regression. It is:

$\sqrt{\alpha_{max}}=b(T-T_{min})(1 - e^{c(T-T_{max})})$

Again, $b$ and $c$ are constants and $T_{min}$ and $T_{max}$ are the minimum and maximum viable growth temperatures. This equation was used in [2] with values of $b=0.032$, $c=0.22$ and minimum and maximum temperatures of 3 and 41 degrees C.

Temperature is very important to maintain, especially if you are trying to optimize production. Be nice to your bugs and they will be nice to you!

[1] De Ory, I., L. Enrique Romero, and D. Cantero. “Modelling the kinetics of growth of Acetobacter aceti in discontinuous culture: influence of the temperature of operation.” Applied Microbiology and Biotechnology 49.2 (1998): 189-193.

[2] Gänzle, Michael G., Michaela Ehmann, and Walter P. Hammes. “Modeling of growth of Lactobacillus sanfranciscensis and Candida milleri in response to process parameters of sourdough fermentation.” Applied and environmental microbiology 64.7 (1998): 2616-2623.

[3]Ratkowsky, D. A., et al. “Model for bacterial culture growth rate throughout the entire biokinetic temperature range.” Journal of bacteriology 154.3 (1983): 1222-1226.

## One Comment on “Temperature and Speed for Bacterial Fermentation”

1. Thank you, really useful. I wondered why my red wine vinegar took 25 weeks to get to 5% (started ~9% ABV) – average temp in the room about 15° C. The current batch, now it’s 20+°C should be quicker. Your instruction paper for mother has answered all my other questions. Delighted to find your site!